Tag Archives: what is the function of primers in PCR

Quantitative/Real-time PCR (Polymerase Chain Reaction)

By | July 6, 2021

PCR (Polymerase Chain Reaction): The two strands of the target DNA molecule are denatured and separated by heating (94 degrees Celsius), in the first step of PCR. In the second step of PCR, annealing, unique sequences around 20 bp (oligonucleotides) complementary to 5′ and 3′ part of the target DNA called primers will bind. The… Read More »

What is PCR – Art of designing primers

By | April 26, 2021

Polymerase Chain Reaction(PCR) is widely held as one of the most important inventions of the 20th century in molecular biology. Small amounts of the genetic material can now be amplified in rather simple manner, thanks to Kerry Mullis invention way back in 1983. Cell free DNA amplification can be performed in many different ways ,which… Read More »