Tag Archives: UAS-GAL4

How to make a recombinant fly line

By | June 14, 2021

Ease of doing genetic experiments makes Drosophila an excellent model system to study development and disease. The reasons for ease in setting up genetic crosses and its subsequent interpretation of results can be attributed to many efficient genetic tools, balancer chromosomes, innumerable genetic markers with clear visible phenotype, mutants for many genes and less number… Read More »

Gal4-UAS system and effect of temperature

By | June 6, 2021

GAL4- UAS system is definitely one of the widely used genetic tool for targeted gene expression in Drosophila. It can be used for both gain of function and loss of function genetic experiments. The wide applicability and everyday use of the system can be attributed to its simple design and availability of lots of reagents… Read More »

Advantages of Drosophila as a model for basic and applied research

By | April 19, 2021

Drosophila has always served as a genetic model system, which was used to understand various aspects of life. This is largely due to some key aspects of fruit fly biology like its fast development cycle, ease and cheap to culture in the lab, wide array of genetic tool ( various mutants available, markers, presence of… Read More »

Over Expression in Drosophila – The UAS-GAL4 Binary System

By | April 15, 2021

The reason for Drosophila melanogaster being a very successful model system when compared to other equally beautiful model organisms in the field will be its short life cycle, cheap to maintain in lab, availability of BALANCER CHROMOSOME’S , many offspring’s, feature-rich morphology (various genetic markers) and not to forget wide array of genetic tools. The… Read More »