Tag Archives: transgenesis

Site directed Transgenic Drosophila

By | July 8, 2021

Transgenesis in Fruit fly, Drosophila melanogaster has come a long way from the days of landmark discovery of Spradling and Rubin in 1982. Drosophila research is highly dependent on P element-mediated transgenesis, as majority of its genetic tools depends on germline transformation by P- element transformation vector – like UAS-GAL4 system, UAS RNAi, FRT FLP… Read More »

How to know chromosomal location of a transgenic construct by using a genetic cross ?

By | July 1, 2021

When we generate a new transgene, the first thing which needs to be done is to map its chromosomal location. This can be done at molecular level by performing Inverse Polymerase chain Reaction (iPCR) and subsequent sequencing. It is also possible to efficiently map the P-element insertion (transgene) by doing a simple cross genetic crosses… Read More »

Advantages of Drosophila as a model for basic and applied research

By | April 19, 2021

Drosophila has always served as a genetic model system, which was used to understand various aspects of life. This is largely due to some key aspects of fruit fly biology like its fast development cycle, ease and cheap to culture in the lab, wide array of genetic tool ( various mutants available, markers, presence of… Read More »