Tag Archives: techniques

DNA methylation systems

By | July 9, 2021
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The E. coli strains used in the laboratory for cloning and DNA isolation contain three site-specific DNA methylation systems. Modification of DNA using these methylation systems protects the potential restriction enzyme recognition sites from cleavage. This genetic engineering technique sheds light on the sensitivity of restrictions endonuclease towards DNA methylation. Such DNA methylation systems are:… Read More »

How to design primers for PCR (Polymerase Chain Reaction)?

By | June 30, 2021
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Primers will have the following characteristics: Primers will have a sequence length of 17 to 30 nucleotides. This enables unique annealing of the primer to a single sequence of the genome. Their GC content will be at least 50 percent. There should be more GC content towards the 3′ end so that it’s bound strongly… Read More »