Tag Archives: RT-PCR

Quantitative/Real-time PCR (Polymerase Chain Reaction)

By | July 6, 2021
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PCR (Polymerase Chain Reaction): The two strands of the target DNA molecule are denatured and separated by heating (94 degrees Celsius), in the first step of PCR. In the second step of PCR, annealing, unique sequences around 20 bp (oligonucleotides) complementary to 5′ and 3′ part of the target DNA called primers will bind. The… Read More »

RT-PCR and Significance of CT value

By | April 23, 2021
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Everywhere we hear about RT-PCR test these days. Reverse Transcription Polymerase chain reaction (RT PCR) test is required to confirm , if one is Covid-19 positive or negative. Different types of PCR : PCR : Polymerase chain reaction is one of widely used molecular biology technique to amplify and detect Deoxy ribo nucleic acid (DNA)… Read More »