Tag Archives: restriction

DNA methylation systems

By | July 9, 2021

The E. coli strains used in the laboratory for cloning and DNA isolation contain three site-specific DNA methylation systems. Modification of DNA using these methylation systems protects the potential restriction enzyme recognition sites from cleavage. This genetic engineering technique sheds light on the sensitivity of restrictions endonuclease towards DNA methylation. Such DNA methylation systems are:… Read More »