Tag Archives: Research

Quantitative/Real-time PCR (Polymerase Chain Reaction)

By | July 6, 2021

PCR (Polymerase Chain Reaction): The two strands of the target DNA molecule are denatured and separated by heating (94 degrees Celsius), in the first step of PCR. In the second step of PCR, annealing, unique sequences around 20 bp (oligonucleotides) complementary to 5′ and 3′ part of the target DNA called primers will bind. The… Read More »

How to design primers for PCR (Polymerase Chain Reaction)?

By | June 30, 2021

Primers will have the following characteristics: Primers will have a sequence length of 17 to 30 nucleotides. This enables unique annealing of the primer to a single sequence of the genome. Their GC content will be at least 50 percent. There should be more GC content towards the 3′ end so that it’s bound strongly… Read More »

What is Epigenetics?

By | June 29, 2021

Genes and behavior Even though identical twins come from the same genetic material, they turn out to be very different people looking alike. So different that one of the twins could get a genetic disorder at the age of 40 and the other could be the most fit person! Why is that so? Epigenetics: The… Read More »

Epigenetic regulation in the developing cerebral cortex/brain

By | June 29, 2021

The cerebral cortex or brain is a complex organ because millions of circuits involving various neuronal subtypes give rise to complex behavior or specific function. This process of neuronal circuit formation involved a complex network of transcriptional control of specification and plasticity of neurons. FEZF2 is one such transcription factor that is essential for the… Read More »