Tag Archives: qPCR

Enhancers and Genetics

By | July 10, 2021

Enhancers They are short DNA fragments with several prominent features such as: driving the expression of the target gene, orientation relative to a target gene promoter, functionally independent of genomic distance, sensitivity towards DNase treatment (since its neucleosome free region), functional influence over chromatin architecture by allowing binding of transcription co-activators and histone acetylation, and… Read More »

Quantitative/Real-time PCR (Polymerase Chain Reaction)

By | July 6, 2021

PCR (Polymerase Chain Reaction): The two strands of the target DNA molecule are denatured and separated by heating (94 degrees Celsius), in the first step of PCR. In the second step of PCR, annealing, unique sequences around 20 bp (oligonucleotides) complementary to 5′ and 3′ part of the target DNA called primers will bind. The… Read More »