How to design primers for PCR (Polymerase Chain Reaction)?
Primers will have the following characteristics: Primers will have a sequence length of 17 to 30 nucleotides. This enables unique annealing of the primer to a single sequence of the genome. Their GC content will be at least 50 percent. There should be more GC content towards the 3′ end so that it’s bound strongly… Read More »
