Tag Archives: PCR

Enhancers and Genetics

By | July 10, 2021

Enhancers They are short DNA fragments with several prominent features such as: driving the expression of the target gene, orientation relative to a target gene promoter, functionally independent of genomic distance, sensitivity towards DNase treatment (since its neucleosome free region), functional influence over chromatin architecture by allowing binding of transcription co-activators and histone acetylation, and… Read More »

Quantitative/Real-time PCR (Polymerase Chain Reaction)

By | July 6, 2021

PCR (Polymerase Chain Reaction): The two strands of the target DNA molecule are denatured and separated by heating (94 degrees Celsius), in the first step of PCR. In the second step of PCR, annealing, unique sequences around 20 bp (oligonucleotides) complementary to 5′ and 3′ part of the target DNA called primers will bind. The… Read More »

How to design primers for PCR (Polymerase Chain Reaction)?

By | June 30, 2021

Primers will have the following characteristics: Primers will have a sequence length of 17 to 30 nucleotides. This enables unique annealing of the primer to a single sequence of the genome. Their GC content will be at least 50 percent. There should be more GC content towards the 3′ end so that it’s bound strongly… Read More »

RT-PCR and Significance of CT value

By | April 23, 2021

Everywhere we hear about RT-PCR test these days. Reverse Transcription Polymerase chain reaction (RT PCR) test is required to confirm , if one is Covid-19 positive or negative. Different types of PCR : PCR : Polymerase chain reaction is one of widely used molecular biology technique to amplify and detect Deoxy ribo nucleic acid (DNA)… Read More »