PCR (Polymerase Chain Reaction): The two strands of the target DNA molecule are denatured and separated by heating (94 degrees Celsius), in the first step of PCR. In the second step of PCR, annealing, unique sequences around 20 bp (oligonucleotides) complementary to 5′ and 3′ part of the target DNA called primers will bind. The… Read More »
Primers will have the following characteristics: Primers will have a sequence length of 17 to 30 nucleotides. This enables unique annealing of the primer to a single sequence of the genome. Their GC content will be at least 50 percent. There should be more GC content towards the 3′ end so that it’s bound strongly… Read More »
Polymerase Chain Reaction(PCR) is widely held as one of the most important inventions of the 20th century in molecular biology. Small amounts of the genetic material can now be amplified in rather simple manner, thanks to Kerry Mullis invention way back in 1983. Cell free DNA amplification can be performed in many different ways ,which… Read More »