Tag Archives: genetic crosses

How to know chromosomal location of a transgenic construct by using a genetic cross ?

By | July 1, 2021

When we generate a new transgene, the first thing which needs to be done is to map its chromosomal location. This can be done at molecular level by performing Inverse Polymerase chain Reaction (iPCR) and subsequent sequencing. It is also possible to efficiently map the P-element insertion (transgene) by doing a simple cross genetic crosses… Read More »

How to make a recombinant fly line

By | June 14, 2021

Ease of doing genetic experiments makes Drosophila an excellent model system to study development and disease. The reasons for ease in setting up genetic crosses and its subsequent interpretation of results can be attributed to many efficient genetic tools, balancer chromosomes, innumerable genetic markers with clear visible phenotype, mutants for many genes and less number… Read More »