Tag Archives: agarose gel

Why a DNA gel is mostly run horizontal and Protein gel’s vertical?

By | May 3, 2021

Why is it always we run our DNA samples in horizontal manner and not horizontal like one employed for proteins. Background: Gel electrophoresis is a technique used for the separation of deoxyribonucleic acid, ribonucleic acid, or protein molecules using an electric current applied to a gel matrix.In most cases the gel is a crosslinked polymer… Read More »

Freeze ‘N Squeeze from Bio-Rad – An alternative to gel extraction kits

By | April 25, 2021

We often tend to use gel elution kits offered by various companies to get our DNA fragment out of agarose gels for cloning and other molecular biology processes. The protocol for eluting DNA using commercial kits is time consuming (even more if u wish to elute multiple bands) and involves toxic chemicals. Here I would… Read More »